Senior Scientific Officer Dr Wu Ji'En

B.S. in Chemistry Peking University • P.R. China • July 1998
Ph.D.  in Natural Product Chemistry, National University of Singapore • Singapore July 2002

Contact Information

The Nuclear Magnetic Resonance Laboratory
The Department of Chemistry, NUS
3 Science Drive 3
Singapore 117543
Office: S8-01-10
E-mail: chmwujie@nus.edu.sg
Tel: +65 6516-4406


Research Interests

  • Study secondary metabolites from microbes for their: (1) structure, (2) function, and (3) regulation

  • Biosynthesis of secondary metabolites: (1) identification of gene clusters, (2) activation of “silent” genes in laboratory condition, and (3) building natural products library.

  • Metabolomic analysis with NMR.

  • Representative Publications

    • J. Lee,# J. Wu,# Y. Deng, J. Wang, C. Wang, J. Wang, F. Tao, C. Chang, Y. Dong, P. Williams, E. P. Greenberg, L.-H. Zhang A cell-cell communication signal integrates quorum sensing and stress response in Pseudomonas aeruginosaNat. Chem. Biol., 2013, 9, 339 - 343. ( highlighted by C&EN in American Chemical Society)
    • Y.-W. He,# J. Wu,# L. Zhou, (F. Yang, B.-L. Jiang, J.-L. Tang, L. Bai, Y. Xu, Z. Deng and L.-H. Zhang, “Xanthomonas campestris diffusible factor is 3-hydroxybenzoic acid and associated with xanthomonadin biosynthesis, cell viability, antioxidant activity and systemic invasionMol. Plant Microbe In., 2011, 24, 948-957.
    • Y. Deng, J. Wu, F. Tao and L.-H. Zhang, “Listening to a new language: DSF family signals modulate quorum sensing in Gram-negative bacteriaChem. Rev., 2011, 111, 160-173.
    • J. Wu,* H.-B. Zhang, J.-L. Xu, R. J. Cox, T. J. Simpson and L.-H.Zhang, “13C labeling reveals multiple amination reactions in biosynthesis of a novel polyketide polyamine antibiotic zeamine from Dickeya zeaeChem. Commun., 2010, 46, 333 –33 5.
    • J. Wu, J. Hothersall, C. Mazzetti, Y. O'Connell, J. A. Shields, A. S. Rahman, R. J. Cox J. Crosby, T. J. Simpson and C. M. Thomas and C. L. Willis, “In vivo mutational analysis of the mupirocin gene cluster reveals labile points in the biosynthetic pathway: the "leaky hosepipe" mechanism.ChemBioChem, 2008, 9, 1500-1508.
    • J. Hothersall, J. Wu, A. S. Rahman, J. A. Shields, S. M. Cooper, E. Stephens, R. J. Cox, J. Crosby, T. J. Simpson and C. M. Thomas, “Mutational analysis reveals that all tailoring region genes are required for production of polyketide antibiotic mupirocin by pseudomonas fluorescens - Pseudomonic acid B biosynthesis precedes pseudomonic acid A” J. Biol. Chem.,2007, 282, 15451-15461.
    • J. Wu, S. M. Cooper, R. J. Cox, J. Crosby, J. Hothersall, T. J. Simpson, C. M. Thomas and C. L. Willis, “Mupirocin H, a novel pseudomonic acid metabolite formed from a truncated polyketide intermediate resulting from mutation of the HMG-CoA synthase analogue, mupH in Pseudomonas fluorescensChem. Commun., 2007, 2040-2042.
    • L.-H. Wang, Y. He, Y. Gao, J. Wu, Y.-H. Dong, C. He, S. Y. Wang, L.-X. Weng, J.-L. Xu, L. Tay, R. X. Fang and L.-H. Zhang “A bacterial cell-cell communication signal with cross kingdom structural analoguesMol. Microbiol., 2004, 51, 903-912.

    #: Co-first authors; *: Corresponding author

    FULL PUBLICATIONS

    • J. Lee,# J. Wu,# Y. Deng, J. Wang, C. Wang, J. Wang, F. Tao, C. Chang, Y. Dong, P. Williams, E. P. Greenberg, L.-H. Zhang “A cell-cell communication signal integrates quorum sensing and stress response in Pseudomonas aeruginosaNat. Chem. Biol., 2013, 9, 339 – 343.

    • Z. Liu,# J. Wu* and D. Huang*New stilbenoids isolated from fungus-challenged black skin peanut seeds and their adipogenesis inhibitory activity in 3T3-L1 cellsJ. Agric. Food. Chem., 2013, DOI: 10.1021/jf400144s.
    • Z. Liu,# J. Wu* and D. Huang*New arahypins isolated from fungal-challenged peanut seeds and their glucose uptake-stimulatory activity in 3T3-L1 adipocytesPhytochem. Lett., 2013, 6, 123 – 127.
    • W. Q. Ong, H. Zhao, C. Sun, J. Wu, Z. Wong, S. F. Y. Li, Y. Hong, and H. Zeng “Patterned Recognitions of Amines and Ammonium Ions by a Pyridine-Based Helical Oligoamide HostChem. Commun., 2012,  48, 6343-6345.
    • S. Ranjit, R. Lee, D.Heryadi, C. Shen, J. Wu, P. Zhang, K.-W. Huang, and X. Liu, “Copper-Mediated C–H Activation/C–S Cross-Coupling of Heterocycles with ThiolsJ. Org. Chem., 2011, 76, 8999–9007.
    • D. Wu, S. C. Lim, Y. Dong, J. Wu, F. Tao, L. Zhou, L.-H. Zhang and H. Song, “Structural basis of substrate binding specificity revealed by the crystal structures of polyamine receptors SpuD and SpuE from Pseudomonas aeruginosaJ. Mol. Biol.,2012, 416, 697-712.
    • J. Zhou, H.-B. Zhang, J. Wu, Q. Liu, P. Xi, J. Lee, J. Liao, Z. Jiang and L.-H. Zhang, “A novel multidomain polyketide synthase is essential for zeamine production and virulence of Dickeya zeaeMol. Plant Microbe In., 2011, 24, 1156-1164.
    • Y.-W. He,# J. Wu,# L. Zhou, F. Yang, B.-L. Jiang, J.-L. Tang, L. Bai, Y. Xu, Z. Deng and L.-H. Zhang, “Xanthomonas campestris diffusible factor is 3-hydroxybenzoic acid and associated with xanthomonadin biosynthesis, cell viability, antioxidant activity and systemic invasionMol. Plant Microbe In., 2011, 24, 948-957.
    • R. W. Scott, A. C. Murphy, J. Wu, J. Hothersall, R. J. Cox, T. J. Simpson, C. M. Thomas and C. L. Willis, “Mupirocin F: Structure Elucidation, Synthesis and RearrangementsTetrahedron, 2011, 67, 5098-5106.
    • J. Hothersall, A. C. Murphy, Z. Iqbal, G. Campbell, E. R. Stephens, J. Wu, H. Cooper, S. Atkinson, P. Williams, J. Crosby, C. L. Willis, R. J. Cox, T. J. Simpson and C. M. Thomas, “Manipulation of quorum sensing regulation in Pseudomonas fluorescens NCIMB 10586 to increase mupirocin productionAppl. Microbiol. Biotech., 2011, 90, 1017-1026.
    • Y. Deng, J. Wu, F. Tao and L.-H. Zhang, “Listening to a new language: DSF family signals modulate quorum sensing in Gram-negative bacteriaChem. Rev., 2011, 111, 160-173.
    • S.-W. An, J. Wu and L.-H. Zhang, “Modulation of Pseudomonas aeruginosa biofilm dispersal by a cyclic-di-GMP phosphodiesterase with a putative hypoxia sensing domainAppl. Environ. Microbiol., 2010, 76, 8160-8173.
    • Y.-W. He,# J. Wu,# J.-S. Cha and L.-H. Zhang, “Rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae produces multiple DSF-family signals in regulation of virulence factor productionBMC Microbiol., 2010, 10:187.
    • Y. Deng,# J. Wu,# L. Eberl and L.-H. Zhang, “Structural and functional characterization of DSF-family quorum sensing signals produced by members of Burkholderia cepacia complexAppl. Environ. Microbiol., 2010, 76, 4675-4683.
    • H. Xu, J. Wu, C.-H. Chen, L.-H. Zhang and K.-L. Yang, “Detecting hydrogen sulfide by using transparent polymer with embedded CdSe/CdS quantum dots.” Sensors and Actuators, B: Chemical, 2010, B143, 535-538.
    • J. Wu,* H.-B. Zhang, J.-L. Xu, R. J. Cox, T. J. Simpson and L.-H.Zhang, “13C labeling reveals multiple amination reactions in biosynthesis of a novel polyketide polyamine antibiotic zeamine from Dickeya zeaeChem. Commun., 2010, 46, 333 –33 5.
    • J. Wu, J. Hothersall, C. Mazzetti, Y. O'Connell, J. A. Shields, A. S. Rahman, R. J. Cox J. Crosby, T. J. Simpson and C. M. Thomas and C. L. Willis, “In vivo mutational analysis of the mupirocin gene cluster reveals labile points in the biosynthetic pathway: the "leaky hosepipe" mechanism.ChemBioChem, 2008, 9, 1500-1508.
    • J. Hothersall, J. Wu, A. S. Rahman, J. A. Shields, S. M. Cooper, E. Stephens, R. J. Cox, J. Crosby, T. J. Simpson and C. M. Thomas, “Mutational analysis reveals that all tailoring region genes are required for production of polyketide antibiotic mupirocin by pseudomonas fluorescens - Pseudomonic acid B biosynthesis precedes pseudomonic acid A” J. Biol. Chem.,2007, 282, 15451-15461.
    • J. Wu, S. M. Cooper, R. J. Cox, J. Crosby, J. Hothersall, T. J. Simpson, C. M. Thomas and C. L. Willis, “Mupirocin H, a novel pseudomonic acid metabolite formed from a truncated polyketide intermediate resulting from mutation of the HMG-CoA synthase analogue, mupH in Pseudomonas fluorescensChem. Commun., 2007, 2040-2042.
    • L.-X. Weng, L.-H. Wang, J.-L. Xu, J. Wu, Q. Li, R. G. Birch and L.-H. Zhang “Molecular and conformational basis of specific and high affinity interaction between AlbA and albicidin antibioticsAppl. Environ. Microbiol., 2005, 71, 1445-1452.
    • L.-H. Wang, Y. He, Y. Gao, J. Wu, Y.-H. Dong, C. He, S. Y. Wang, L.-X. Weng, J.-L. Xu, L. Tay, R. X. Fang and L.-H. Zhang “A bacterial cell-cell communication signal with cross kingdom structural analoguesMol. Microbiol., 2004, 51, 903-912.
    • J. Wu, X.-F. Cheng, L. J. Harrison, S. H. Goh and K. Y. Sim “A phloroglucinol derivative with a new carbon skeleton from Hypericum perforatum (Guttiferae)Tetrahedron Lett., 2004, 45, 9657-9659.
    • Z. L. Liu, Y. J. Xu, J. Wu, S. H. Goh and S. H. Ho “Feeding Deterrents from Dictamnus dasycarpus Turcz Against Two Stored-Product InsectsJ. Agric. Food Chem., 2002, 50, 1447-1450.
    • J. Wu, Y. J. Xu, X. F. Cheng, L. J. Harrison, K. Y. Sim, S. H. Goh “A highly rearranged tetraprenylxanthonoid from Garcinia gaudichaudii (Guttiferae)Tetrahedron Lett.,  2001, 42, 727-729.

    PATENTS

  • P3. Y. Deng, J. Wu, and L.-H. Zhang “Novel antimicrobial compounds and uses thereof in treatment of bacterial and fungal infections” PCT Int. Appl. 2011, WO 2011142724 A1 20111117

  • P2. J. Wu, H.-B. Zhang and L.-H. Zhang “Novel polyamino polyketide antibiotics produced by Dickeya zeae” PCT Int. Appl. 2010, 46 pp. CODEN: PIXXD2 WO 2010053455 A1 20100514 CAN 152:546694 AN 2010:597859 CAPLUS.

  • P1. L.-H. Wang, J. Wu, and L.-H. Zhang “Production of core/shell semiconductor nanocrystals in aqueous solutions” PCT Int. Appl. 2006, 44 pp. CODEN: PIXXD2 WO 2006054952 A1 20060526 CAN 144:499935 AN 2006:494553 CAPLUS.

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