Professor Thorsten WOHLAND

(Vice Dean - Research and Space)




Dipl. Phys., University of Heidelberg, Germany; PhD, Swiss Federel Institute of Technology at Lausanne (EPFL, Switzerland); Postdoctoral., Stanford University (USA).

Contact Information

Office: S1A-02-12
Tel: (65)-6516-1248 | Fax: (65)-6776-7882
Email: twohland@nus.edu.sg | Personal webpage


 

ORCID:  0000-0002-0148-4321
ResearcherID: H-8285-2012

 

Research Interests

My group works on the development of new fluorescence spectroscopy techniques for the measurement of biomolecular interactions in cells, tissues, and organisms. Specific foci are:

  • Techniques: Single wavelength excitation fluorescence cross-correlation spectroscopy (SW-FCCS) and imaging fluorescence correlation spectroscopy (ITIR-FCS, SPIM-FCS).
  • Biological systems: Transmembrane protein receptors and peptides and their interaction with membranes, measurement of biomolecular actions and interactions in living cells and organisms.

 

Research Highlight

In recent years our group focused on developing image based fluorescence tech-niques in which the signal at each pixel of a camera is evaluated to determine sample structure, dynamics, and interactions and single molecule sensitivity. We have implemented these techniques on total internal reflection (TIR) and single plane illumination microscopes (SPIM). Abbreviations: FCS – fluorescence correlation spectroscopy; FCCS – fluorescence cross-correlation spectroscopy; N&B – number and brightness analysis; FRAP – Fluorescence Recovery After Photobleaching; SRRF – super-resolution radial fluctuation; SPT – single particle tracking; spFRET – single particle Förster resonance energy transfer.

 

Representative Publications   

  • Marzinek, J.K.; Bag, N.; Huber, R.G.; Holdbrook, D.A.; Wohland, T.; Verma, C.S.; Bond, P. J. A Funneled Conformational Landscape Governs Flavivirus Fusion Peptide Interaction with Lipid Membranes. J. Chem. Theory Comput. 2018, 14, 3920-3932.
  • Ng, J.; Kamm, R.D.; Wohland, T.; Kraut, R.S. Evidence from ITIR-FCS Diffusion Studies that the Amyloid-Beta (Aβ) Peptide Does Not Perturb Plasma Membrane Fluidity in Neuronal Cells. J. Mol. Biol. 2018, 430, 3439-3453.
  • Sankaran, J.; Karampatzakis, A.; Rice, S.A.; Wohland, T. Quantitative imaging and spectroscopic technologies for microbiology. FEMS Microbiol. Lett. 2018, 365 (9). 
  • Harwardt, M.-L.E.; Dietz, M.S.; Heilemann, M.; Wohland, T. SPT and Imaging FCS Provide Complementary Information on the Dynamics of Plasma Membrane Molecules. Biophys J. 2018, 114, 2432-2443.
  • Hoischen, C.; Yavas, S.; Wohland, T.; Diekmann, S. CENP-C/H/I/K/M/T/W/N/L and hMis12 but not CENP-S/X participate in complex formation in the nucleoplasm of living human interphase cells outside centromeres. PLoS One 2018, 13(3).
  • Wang, J.; Yin Y.; Lau S.; Sankaran, J.; Rothenberg, E.; Wohland, T.; Meier-Schellersheim, M.; Knaut, H. Anosmin1 Shuttles Fgf to Facilitate Its Diffusion, Increase Its Local Concentration, and Induce Sensory Organs. Dev. Cell 2018, 46, 1–16.