Professor YAO Shao Qin




B.Sc., Ohio State University (USA); PhD, Purdue University (USA); Postdoctoral, University of California Berkeley/ Scripps Research Institute (USA).

Contact Information

Office: S5-03-05
Tel: (65)-6516-2669 | Fax: (65)-6779-1691
Email: chmyaosq@nus.edu.sg | Personal webpage


 

ORCID: 0000-0003-4715-769X
ResearcherID: B-8825-2008   

 

Recognition and Achievements

  • Member of the International Scientific Advisory Board, Angewandte Chemie,
  • Dean’s Chair Professor, National University of Singapore, 2009-2012.
  • ASAIHL-Scopus Young Scientist Award, International, 2008
  • Outstanding Scientist Award, Faculty of Science, National University of Singapore, Singapaore, 2007
  • Mr & Mrs Sun Chan Memorial Award, International, 2006
  • Young Research Award, National University of Singapore, 2005
  • Young Investigator Award, A*STAR Singapore, 2002

 

Research Interests

“Catalomics” - We are interested in developing powerful strategies in Chemical Biology and Chemical Proteomics that enable organism-wide, high-throughput studies of enzymes. Specific foci are:

  •  Chemical Biology
  •  Medicinal Chemistry

 

Research Highlight

Ref. : Qian, L.; Fu, J.; Yuan, P.; Du, S.; Huang, W.; Li, L.; Yao, S. Q. Intracellular Delivery of Native Proteins Facilitated by Cell-Penetrating Poly(disulfide)s. Angew. Chem. Int. Ed.201857, 1532-1536(selected as cover). Intracellular delivery of therapeutic proteins is highly challenging and in most cases requires chemical or genetic modifications. Herein, two complementary approaches for endocytosis-independent delivery of proteins to live mammalian cells are reported. By using either a “glycan” tag naturally derived from glycosylated proteins or a “traceless” tag that could reversibly label native lysines on non-glycosylated proteins, followed by bioorthogonal conjugation with cell-penetrating poly(disulfide)s (CPDs), we achieved intracellular delivery of proteins (including antibodies and enzymes) which, upon spontaneous degradation of CPDs, led to successful release of their “native” functional forms with immediate bioavailability.

 

Representative Publications   

  • Qian, L.; Fu, J.; Yuan, P.; Du, S.; Huang, W.; Li, L.; Yao, S. Q. Intracellular Delivery of Native Proteins Facilitated by Cell-Penetrating Poly(disulfide)s. Angew. Chem. Int. Ed.201857, 1532-1536.
  • Mao, X.; Yuan, P.; Yu, C.; Li, L.; Yao, S.Q. Nanoquencher-Based Selective Imaging of Glutathione and Protein Glutathionylation in Live Mammalian Cells. Angew. Chem. Int. Ed., 2018, 57, 10257-10262.
  • Pan, S.; Jang, S.-Y.; Liew, S. S.; Fu, J.; Wang, D.; Lee, J.-S.; Shao Q. Yao. A Vinyl Sulfone-Based Fluorogenic Probe Capable of Selective Labelling of PHGDH in Live Mammalian Cells. Angew. Chem. Int. Ed., 2018, 57, 579-583.
  • Yuan, P.; Zhang, H.; Qian, L.; Mao, X.; Du, S.; Yu, C.; Peng, B.; Yao, S.Q. Intracellular Delivery of Functional Native Antibodies under Hypoxic Conditions by Using a Biodegradable Silica Nanoquencher. Angew. Chem. Int. Ed.2017,  56, 12481-12485.
  • Wang, D.; Du, S.; Cazenave-Gassiot, A.; Ge, J.; Lee, J.-S.; Wenk, M.; Yao, S.Q. Global Mapping of Protein-Lipid Interactions by Using Modified Choline-Containing Phospholipids Metabolically Synthesized in Live Cells. Angew. Chem. Int. Ed., 2017,56, 5829-5833.
  • Peng, B.; Thorsell, A.-G.; Karlberg, T.; Schüler, H.; Yao, S.Q.  Small Molecule Microarray (SMM)-Based Discovery of PARP14 Inhibitors. Angew. Chem. Int. Ed, 2017, 56, 248-253.
  • Ge, J.; Zhang, C.-W.; Ng, X.W.; Peng, B.; Pan, S.; Du, S.; Wang, D.; Li, L.; Lim, K.-L.; Wohland, T.; Yao, S.Q., “A Suite of Puromycin Analogs Capable of Multiplexed Imaging and Profiling of Protein Synthesis and Dynamics in Live Cells and Neurons”, Angew. Chem. Int. Ed., 2016, 55, 4933-4937. 
  • Li, Z.; Qian, L.; Li, L.; Bernhammer, J. C.; Huynh, H. V.; Lee, J.-S.; Yao, S. Q. Tetrazole Photoclick Chemistry: Reinvestigating Its Suitability as a Bioorthogonal Reaction and Potential Applications. Angew. Chem. Int. Ed.2016, 55, 2002-2006.
  • Fu, J.; Yu, C.; Li, L.; Yao, S.Q. Intracellular Delivery of Functional Proteins and Native Drugs by Cell-Penetrating Poly(disulfide)s. J. Am. Chem. Soc. 2015, 137, 12153-12160.
  • Li, L.; Zhang, C. W.; Chen, G. Y. J.; Zhu, B. W.; Chai, C.; Xu, Q. H.; Tan, E. K.; Zhu, Q.; Lim, K. L.; Yao, S. Q. A Sensitive Two-Photon Probe to Selectively Detect Monoamine Oxidase B Activity in Parkinson's Disease Models. Nat. Commun20145, 10.