Assistant Professor ZHAO Ziqing (Winston)

(NUS Presidential Young Professorship)




B.Sc. (honors) in Chemistry and Biology, Caltech, 2004–2008;
Ph.D. in Biophysics, Harvard University, 2009–2015;
Research Fellow, Institute of Molecular and Cell Biology and Genome Institute of Singapore, A*STAR, 2015–2019

Contact Information

Office: S1A-02-13
Tel: (65)-6516 4384
Email: zhaozw@nus.edu.sg | CV


 

ORCID: 0000-0002-1233-9867
ResearcherID: M-8960-2019
Google Scholar: https://scholar.google.com.sg/citations?hl=en&user=KHOh00MAAAAJ

 

Recognition and Achievements

  • NUS Presidential Young Professorship, NUS, 2019
  • Selected Delegate of 65th Lindau Nobel Laureate Meeting, Germany, 2015
  • National Science Scholarship, A*STAR, 2004–2015                                                                                                     
  • Cold Spring Harbor Asia Poster Award (2nd Prize), Cold Spring Harbor Asia, 2013
  • Student Research Achievement Award, The Biophysical Society, 2013
  • Dudley R. Herschbach Teaching Award, Harvard University, 2012
  • Richard P. Schuster Memorial Prize, Caltech, 2008
  • Phi Tau Phi Scholastic Honor Society of America Scholarship, 2007
  • Summer Undergraduate Research Fellowship, Caltech, 2006, 2007
  • Upper Class Merit Award (Carnation Scholarship), Caltech, 2006, 2007

 

Research Interests

The research in my group focuses on the development and application of advanced imaging-based approaches to quantitatively probe the biophysical chemistry of chromatin dynamics at the single-cell level. In particular, we are interested in understanding how molecular processes related to chromatin structure, accessibility, and expression are organized and regulated in space and time. By integrating approaches from optical microscopy and spectroscopy, genome and protein engineering, and computational analysis, our work aims to illuminate the physico-chemical driving forces that underpin the spatio-temporal heterogeneities in chromatin dynamics, as well as the physiological implications of their misregulation in human diseases.

 

Research Highlights

We have developed a series of novel imaging techniques that overcome the critical challenges posed by the high molecular density and fluorescence background in the mammalian cell nucleus. As such, they have enabled single-molecule and super-resolution imaging of key cell nuclear structures, as well as the monitoring of highly transient DNA–protein interactions with superior sensitivity in complex systems such as live embryos. Using these techniques, we mapped the spatial organizations of gene transcription and DNA replication at sub-diffraction-limit resolution, and revealed critical temporal changes in the binding dynamics of transcription factors that predict cell fates over the course of early embryonic development. These works pave the way for the quantitative dissection of diverse molecular processes that modulate the human genome at unprecedented levels of detail, and shed important light on the hierarchical landscape that regulates their spatio-temporal dynamics in vivo.

 

Representative Publications  

  • Su, Q. P.*, Zhao, Z. W.*, Meng, L., Ding, M., Zhang, W., Li, Y., Liu, M., Li, R., Gao, Y.-Q., Xie, X. S., Sun, Y. CTCF-organized chromatin structures mediate the spatio-temporal propagation of replication foci. eLife (in review) (*: equal contribution).
  • Zhao, Z. W.*, White, M. D.*, Alverez, Y. D.*, Zenker, J.*, Bissiere, S., Plachta, N. Quantifying transcription factor–DNA binding in single cells in vivo with photoactivatable fluorescence correlation spectroscopy. Nature Protoc. 12:1458–1471 (2017) (*: equal contribution).
  • White, M. D., Angiolini, J. F., Alverez, Y. D., Kaur, G., Zhao, Z. W., Mocskos, E., Bruno, L., Bissiere, S., Levi, V., Plachta, N. Long-lived binding of Sox2 to DNA predicts cell fate in the four-cell mouse embryo. Cell 165:75–87 (2016) (cover article).
  • Zhao, Z. W., Xie, X. S., Ge, H. Nonequilibrium relaxation of conformational dynamics facilitates catalytic reaction in an elastic network model of T7 DNA polymerase. J. Phys. Chem. B 120:2869–2877 (2016).
  • Zhao, Z. W.*, Roy, R.*, Gebhardt, J. C. M.*, Suter, D. M.*, Chapman, A. R., Xie, X. S. Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy. Proc. Natl. Acad. Sci. U.S.A. 111:681–686 (2014) (*: equal contribution).
  • Zhao, Z. W., Gebhardt, J. C. M., Suter, D. M., Xie, X. S. Reply to “Convergence of chromatin binding estimates in live cells”. Nature Methods 10:692 (2013).
  • Gebhardt, J. C. M., Suter, D. M., Roy, R., Zhao, Z. W., Chapman, A. R., Basu, S., Maniatis, T., Xie, X. S. Single-molecule imaging of transcription factor binding to DNA in live mammalian cells. Nature Methods 10:421–426 (2013).