Mass Spectrometry Laboratory

Mass spectrometry offers the capability to identify unknown compounds, quantify known materials and characterize structure and properties of molecules.

Services Available

Nominal Mass Analysis

Produce full mass spectra with low resolution using the following ionization techniques:

EI	- Electron Ionization
ESI	- Electrospray Ionization
APCI	- Atmospheric Pressure Chemical Ionization
FAB	- Fast Atom Bombardment
MALDI	- Matrix Assisted Laser Desorption Ionization

Accurate Mass Measurement

To confirm or determine the elemental composition of compounds using high resolution measurement of selected ions with <5ppm accuracy.

Separation Techniques with Mass Spectrometry

GC/MS - To separate and identify the components of volatile mixture with EI
LC/MS – To separate compounds of non volatile or polar mixtures with ESI or APCI.

Tandem Mass Spectrometry

To obtain fragmentation for structural determination by MSⁿ

Instruments

	Bruker micrOTOFQII
	Bruker amaZonX
	Bruker Autoflex III TOF/TOF
	Finnigan MAT95XL
	Finnigan LCQ
	Finnigan TSQ7000
	Agilent 5975 GCMSD
	Agilent 5975 DIPMS

Bruker micrOTOFQII	The MicrOTOF-QII is an accurate mass, high resolution instrument for small molecule identification as well as intact protein work. Coupled to an Dionex Ultimate 3000 RSLC system and Kd Science Syringe pump infusion system Capability: ESI, APCI and MS/MS Mass range: 50-20,000 m/z Resolution: up to 17,500 FWHM Mass accuracy: <2ppm with internal calibration; <5ppm with external calibration.
Bruker amaZonX	The AmaZon X utilizes unique spherical ion trap architecture in combination with dual ion funnel transfer technology for MSn analysis of complex samples. Coupled to an Dionex Ultimate 3000 RSLC system and Kd Science Syringe pump infusion system Capability: ESI, APCI and MSn Mass range: 50-3000 m/z Resolution: 0.1u FWHM
Bruker Autoflex III TOF/ TOF	The Autoflex III TOF/TOF is a powerful plateform for many MALDI based applications including protein sequencing, protein analysis and polymer analysis. MALDI-TOF/TOF with Linear and Reflectron analyzers Mass range : up to 300,000 m/z Resolution: up to 26,000 FWHM in Reflectron mode Laser type: Smartbeam laser.
Finnigan MAT95XL	Finnigan MAT95XL is a high resolution double focusing mass spectrometer with BE geometry. Coupled to an Agilent 6890A Gas Chromatograph With direct insertion probe for EI With Cs-Gun and FAB probe for FAB Capability: EI, FAB Mass range: 10-3500 m/z at full accelerating voltage Maximum resolution: up to 60,000 FWHM .
Finnigan LCQ	Finnigan LCQ is a benchtop quadrupole Ion trap mass spectrometer Coupled to an SpectraSystem HPLC system With divert inject valve for ESI and APCI With infusion pump for ESI and APCI Capability: ESI, APCI, MSn Mass range: 50-2000 m/z Resolution: Unit mass resolution.
Finnigan TSQ7000	Finnigan TSQ7000 is a triple stage quadrupole mass spectrometer. Coupled to an SpectraSystem HPLC system With divert inject valve for ESI and APCI With infusion pump for ESI and APCI With direct insertion probe for EI Capability: EI, ESI, APCI mass range: 50-2000 m/z, 200-4000 m/z Resolution: Unit mass resolution
Agilent 5975 GCMSD	Agilent 5975 is a low resolution quadrupole benchtop mass spectrometer. Coupled to an Agilent 7890A Gas Chromatograph With NIST Mass Spectra Library Capability: EI Mass range: 10-1050 m/z Resolution: Unit mass resolution
Agilent 5975 DIPMS	Agilent 5975 is a low resolution quadrupole benchtop mass spectrometer. Coupled to an SIS Direct Insertion Probe Capability: EI Mass range: 10-1050 m/z Resolution: Unit mass resolution

Analysis Requests

There are three different forms available for download.

Form 1 for pure compound analysis (EI, CI, FAB, ESI, APCI, MS/MS).

Form 2 for GC/MS analysis (EI and CI).

Form 3 is for LC/MS analysis (ESI and APCI).

All requests MS for analysis should be sent via chmwlk@nus.edu.sg .

Click here for more information on submission of sample and collection of results.

Click here for more information on selection on ionization technique.